Faculty of Engineering and Science School of Science ANALYTICAL TECHNIQUES and QA/QC PRINCIPLES OMED 0104 CHARACTERISATION OF AN UNKNOWN SAMPLE BY MASS SPECTROMETRY Name: CHARMI THAKKAR ID number: 001143170 Laboratory Group: 11 This submission is part of the Lab component of the OMED0104 module TITLE: CHARACTERISATION OF AN UNKNOWN SAMPLE BY MASS SPECTROMETRY AIM: INTRODUCTION: INSTRUMENTS USED:For GC-MS experiment INSTRUMENT: Agilent 6890-5973 Gas-Chromatography Quadrupole MSD SYRINGE: 10 µL Hamilton Syringe OPERATING SOFTWARE: ChemStation GC COLUMN: DB-5MS (0.25 mm I.D x 30 m Length; FILM THICKNESS of 0.25 µm) CARRIER GAS: Helium For LC-MS experiment INSTRUMENT USED: Waters Acquity H-Class Ultra-Performance Liquid Chromatography Quadrupole MSD OPERATING SOFTWARE: MassLynx 4.1 CHROMATOGRAPHIC COLUMN: Acquity UPLC BEH C18 (modifier) 1.7µm (pore size) 2.1 mm I.D x 50 mm Length CHEMICAL USED: dichloromethane, methanol, formic acid, distilled water. PROCEDURE: EVALUTION OF RESULT: DICUSSION: CONCLUSION: QUESTIONS: 1. Explain what you understand by negative ion detection and positive ion detection used in analysing your samples by ESI MS? 2. From your recorded mass spectra try to elucidate the identity of your unknown samples. Please give a full explanation to your reasoning of your results. 3. In this experiment you have used the ESI and EI ionisation techniques. Using your knowledge of MS, explain what you know about the ionisation technique known as CI? . LEARNING OUTCOMES: REFERENCE:

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